3 replies to this topic

[zhglu]

hi all,

I'm now need one protein that could tightly bind to plasmid DNA. but many DNA-binding proteins only bind to specific DNA sequence. It heard that NLS-containg proteins could bind to DNA by charge interaction, i tried some, none could bind tightly.

thanks

[phdconsult]

Try commercially available histones from Sigma- these proteins are rather non-specific. Good luck

[phdconsult]

Also, the beta or alpha subunit of RNA polymerase could be used - pretty high binding constants and entirely non specific without the sigma factor.

[zhglu]

Thank you very much!