Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

DNA binding proteins


  • Please log in to reply
3 replies to this topic

#1 zhglu

zhglu

    member

  • Active Members
  • Pip
  • 7 posts
0
Neutral

Posted 17 May 2004 - 12:13 AM

hi all,

I'm now need one protein that could tightly bind to plasmid DNA. but many DNA-binding proteins only bind to specific DNA sequence. It heard that NLS-containg proteins could bind to DNA by charge interaction, i tried some, none could bind tightly.

thanks

#2 phdconsult

phdconsult

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 89 posts
0
Neutral

Posted 17 May 2004 - 04:59 AM

Try commercially available histones from Sigma- these proteins are rather non-specific. Good luck

#3 phdconsult

phdconsult

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 89 posts
0
Neutral

Posted 17 May 2004 - 10:45 AM

Also, the beta or alpha subunit of RNA polymerase could be used - pretty high binding constants and entirely non specific without the sigma factor.

#4 zhglu

zhglu

    member

  • Active Members
  • Pip
  • 7 posts
0
Neutral

Posted 17 May 2004 - 08:08 PM

Thank you very much!




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.