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Which epitope tag to use for ChIP?

Epitope Tag Cloning Chip Fusion Protein

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#1 Epigeneticist

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Posted 09 January 2014 - 02:12 PM

From your experience, which epitope tag worked the best for ChIP? I am going to tag my protein but I also would like that epitope tag to be suitable for ChIP. I have thought about Flag, Myc, HA, V5, His, or maybe you have another tag that worked for you. Please explain why the epitope tag worked poorly or well. 
 
Note: I understand the antibody plays a major role.
 
Thanks!
 


#2 Epigeneticist

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Posted 13 January 2014 - 03:21 PM

Has anyone tagged their protein with HA or Flag and done ChIP PCR or ChiP seq? Comments? Suggestions?







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