Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Total Protein in COS-7 Cells

  • Please log in to reply
1 reply to this topic

#1 alphawhiskey



  • Active Members
  • Pip
  • 13 posts

Posted 09 January 2014 - 01:06 PM

Hi all,


How much total protein should I be expecting from COS-7 cells? 


I grew cells in a 6-well plate to 80-90% confluency, scraped and collected the cells in PBS, spun them down and resuspended in 100uL of Tris and NaCl buffer and sonicated this. My total protein yield was only about 0.25ug of total protein per uL of lysate. Is this normal? How much protein should I expect from each well?



#2 bob1


    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,511 posts

Posted 09 January 2014 - 03:25 PM

A confluent well of a 6 well plate should contain about 1 million cells which should give you somewhere between 1-10 ug of protein but this is cell type dependent.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.