I would like to purify low abundant protein from protein extract. I bind policlonal antibodies of this protein to magnetic beads from Invitrogen.
The protein extract consists 7M urea/2M thiourea/1% DTT. The elution buffer proposed by Invitrogen (cytric acid) is not working. Maybe solution with higher urea cc? Do you any ideas how to purify protein in such conditions?
Thanks in advance!