I am trying to lyse COS-7 cells to detect actin-related proteins in the cytoplasm. My original lysis buffer recipe is:
50mM Tris pH 8.0
Benzamidine HCl 1mM
However, because the Triton-X reacts with Bradford, should I reduce it to 0.05% Triton-X? Would that be enough to lyse mammalian cells?