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DNA from frozed cells???


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#1 ppen

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Posted 07 January 2014 - 03:35 AM

Hi

 

Please, can you help me. Is it possible to extract DNA from frozed cells. I would melt the cells. Fuge the cells the bottom of the tube, wash them once with PBS, and make DNA isolationaccording to the DNA isolation- kit. Does DMSO (used in the freezed cells) bother DNA analysis?

 

 

 



#2 GNANA

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Posted 07 January 2014 - 08:28 AM

Yes you can extract DNA from frozen cells, just pellet them out after thawing take out the supernatant and start with kit protocol.  washing is optional and i dont think the left over DMSO do harm further steps or DNA.


I would prefer being perfectionist rather than a passionist in Research.

I always had an alternate hypothesis....

#3 ppen

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Posted 10 January 2014 - 12:34 AM

Thank you for your response!!!






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