Okay this sounds a bit dumb but all these are new to me, so please help in anyway you can.
I'm trying to design a new primers (let's say for a bacteria). I know for amplicons you're suppose to select 75-100bp, avoid secondary structures etc...
but how do you go about selecting the target sequence? Is there some kind of database where you can see the dna sequence of a bacteria and can select a target sequence/amplicons on that? and thus the primers.
I'm sorry if I'm not being clear...please any help would be much appreciated.
Edited by ntsalihah, 06 January 2014 - 04:02 AM.