Any help with the question below will be much appreciated! Thanks in advance!
I have a buffer I use for digesting DNA. It is composed of the following:
100mM Tris-HCl pH 7.4
I want to remove magnesium in this assay and substitute for calcium. Thus, can I simply use 30mM CaCl2 instead of 30mM MgCl2? Or do I need to consider the difference in molecular weight between calcium and magnesium and adjust the final concentration?