I have been trying to make M63 minimal media using a standard recipe that is consistent across a number of protocol manuals and online sources. Below is the recipe I have been trying:
For 1 liter of medium add:
MilliQ water to 1L
Adjust to pH 7 using KOH
Add 1mL 1M MgSO4
All seems to go well until I begin to adjust the pH using KOH. As soon as I begin adding KOH the solution begins to turn a dark dingy gray and begins to flocculate a fluffy grayish-brownish precipitate.
Any ideas on what I might be doing wrong or thoughts about how to correct this problem?
Thanks for any help.
Edited by gorbster, 04 January 2014 - 02:36 PM.