I am trying to purify a protease enzyme from Bacillus sp. The predicted pI of the protease is 9.2 and i am using the culture supernatant as the source of the enzyme. I have used ammonium sulfate precipitation and checked the activity of the enzyme
My queries are
1. What ion exchange column can i choose for the ion exchange chromatography? Cation or anion exchange?
2. How can the column packing be done manually? I do not have prepacked columns or FPLC apparatus. Can the packed column be operated using a syringe or peristalitic pump?