Hi every one,
Happy New Year in advance.
I got three samples that they are same in promoter site and suppose to give exactly same sequence as well.
One of them has exactly the sequence that I expect but in the other two I can read the sequence only for 100 nucleotides, which isn’t enough to confirm the part that I am looking for. The strange thing is, the result become unreadable in exactly in same point in these two results.
What I thought might be some thing wrong by these two samples but on the hand I have sequencing of other part of these samples which is absolutely fine so the bad result couldn't be of poor sample.
The other possibility could be primer but the prime works and gave reading of 100 bp.
Any advise what could be the reason?
I thought something might be happened in company that I sent for sequencing.