Hello! I am new in the proteomic area. I need to do a quantitative western blot and use Actin as internal control. I am not sure how to blot probe two proteins in one membrane (my target protein and Actin). Can I cut out the area for Actin (~50kDa) and probe anti-Actin antibody and probe the other area with my target antibody? Or do I need to first probe my target protein and strip and reprobe the Actin?