i would linke to sequence the whole rpoB gene. But how can I do it?
Should I divide the gene into smaller regions, do pcr and sequencing separately?
For example Khamis, Raoult and La Scola (2004) have a set of primers for rpoB.
How can I now select the best primers for my purpose? I thought about following pairs:
1) C35F and C1415F
2) C890F and C2130R
3) C1415F and C2700R
4) C2130F and C3490R
5) C2625F and C3490R
For sequencing I am planing to only use the forward primers.
Please let me know your suggestions. Many thanks!
Edited by tretol, 28 December 2013 - 05:55 AM.