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RNA yield and quality: Trizol vs. Column based

hybrid approach for RNA

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#1 Trys

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Posted 25 December 2013 - 11:58 AM

Hi Friends,

 

I would like to isolate RNA with both good yield and good quality. Tried Trizol and Qiagen column, so far I felt that Trizol had high yield but with limit on RIN value quality (like RIN>9). I wonder whether taking the advantage of Trizol's high yield and do further purification by column method.

 

I appreciate any comments about this hybrid approach on RNA's yield and quality,

 

Thank,

 

Trys



#2 HOYAJM

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Posted 06 January 2014 - 08:53 AM

You could always do a lithium chloride precipitation to clean up the RNA after TRIZOL. I would worry about a substantial loss in yield if you combine these protocols.



#3 Trys

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Posted 07 January 2014 - 10:07 PM

Thank you for your reply, Hoyajm

I will look into this method,






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