I want to check a primer sequence in promoter area of two genes for methylaion analysis:
5' TTGGGAGGGGGCTCGGGCAT 3'
5' CAGAGCTGGCAGCGGACGGT 3'
17-Beta Hydroxysteroid dehydrogenase type 1:
I tried to find them in blast and ensembl, but :
in blast I found but I donot know their position in promoter or not
I found the forward only( BRCA-1 in area not exon may promoter???) ( in 2nd gene in exon)
please tell me " Is that primers right or not and to do they donot give the result I want in methylation analysis using resriction enzyme?"
If that primer is wrong, so what is the solution?