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Dot blot anomaly

dot blot western blot

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#1 Shrubal

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Posted 22 December 2013 - 06:29 AM

I carried out a dot blot for various concentrations of my cell lysate and found weak positive staining for my protein of interest at a concentration of 15-25ng. However there was absolutely no staining at higher lysate concentrations (the positive control stained even at 100ng). Can someone tell me why? Thanks!



#2 Missle

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Posted 23 December 2013 - 05:47 AM

was the positive control a lysate or purified protein?



#3 Shrubal

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Posted 08 January 2014 - 02:03 AM

The positive control was also a whole cell lysate







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