Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

RNA from cultured Osteoblasts


  • Please log in to reply
2 replies to this topic

#1 drdtkk

drdtkk

    member

  • Members
  • Pip
  • 5 posts
0
Neutral

Posted 14 May 2004 - 07:42 AM

We have been trying to extract RNA from cultured primary human osteoblasts using Tri Reagent with very poor yields.

We initially tried 6 well plates but have recently been using 100mm Diameter culture dishes.

Do we need bigger dishes?
Is there anything special about Osteoblasts that we've missed?????

#2 pcrman

pcrman

    Epigenetist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,165 posts
68
Excellent

Posted 19 May 2004 - 05:36 PM

Did you scale up the amount of Tri Reagent added to your dishes? since you said you used to use 6-well plate. Stick to what the manual says about the amount of Tri reagent needed. If it is not enough, you will get low yeild of RNA.

#3 il0postino

il0postino

    member

  • Active Members
  • Pip
  • 23 posts
1
Neutral

Posted 22 May 2004 - 06:18 PM

Don't you grow these things in calcium free medium?




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.