We have been trying to extract RNA from cultured primary human osteoblasts using Tri Reagent with very poor yields.
We initially tried 6 well plates but have recently been using 100mm Diameter culture dishes.
Do we need bigger dishes?
Is there anything special about Osteoblasts that we've missed?????
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#2
pcrman
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Posted 19 May 2004 - 05:36 PM
Did you scale up the amount of Tri Reagent added to your dishes? since you said you used to use 6-well plate. Stick to what the manual says about the amount of Tri reagent needed. If it is not enough, you will get low yeild of RNA.
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il0postino
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Posted 22 May 2004 - 06:18 PM
Don't you grow these things in calcium free medium?
