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polyacrylamide gel with sybr green staining

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#1 mushroom911



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Posted 20 December 2013 - 05:29 AM

My lab is trying to get rid of ethidium bromide. So, i try to stain polyacrylamide gel with sybr green but somehow my dna bands and marker look very blurry. :/

I made the staining solution with 100 ml TBE buffer and 10 microlitre sybr green. I soak the gel in staining solution for 40 minutes and wash it briefly with water after staining. i wonder is there anything that i did wrong?? What i can do to improve the gel picture?

#2 phage434



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Posted 20 December 2013 - 07:47 AM

I assume your gel is run in TBE as well. You want the stain solution and running buffer to be the same (except for dye).

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