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Southern Blotting


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#1 molmicro

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Posted 14 May 2004 - 02:42 AM

Has anyone any experience of using a semi dry blotter for Southern blotting. At present I am tranfering overnight using a weight, I think there is a blotter that is suitable for nucleic acids and can transfer in an hour.
Has anyone used this or know anything about it?

#2 phdconsult

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Posted 30 May 2004 - 05:44 PM

Molmicro

Use the overnight weight method. Don't change it It has stood the test of time for over 10 years. Add the maniatis books to the weight you place on top in the evening, go home and do something fun like sleeping.The semi-dry method has a slight problem- the transfer is asymmetric at times and you get more DNA transferred at one end of the blot.

The books and bricks work better- much better than that semi-dry equipment.




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