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does DNA methylation outside promoter region affect gene Transcription/expressio

DNA methylationpromoter BSP primer design

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#1 Moemedawar

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Posted 18 December 2013 - 04:48 AM

I'm designing BSP primers for DNA methylation for rat BDKRB1 gene. Since gene methylation in promoter region has a regulatory effect on gene transcription, I looked up promoter regions for the gene and I did CpG island prediction to find islands. However, no CpG islands were found in the promoter region. Moreover, BSP primer design criteria entail that the primer pair must span a gene target with maximal number of islands without containing a CpG island in primer sequence itself.

 

question is:

 

Is there a use in looking at methylation status for gene expression inside the transcribed region? does DNA methylation outside promoter region affect gene transcription/expression?


Edited by Moemedawar, 18 December 2013 - 08:16 AM.


#2 rakun

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Posted 11 January 2014 - 08:29 AM

I'm designing BSP primers for DNA methylation for rat BDKRB1 gene. Since gene methylation in promoter region has a regulatory effect on gene transcription, I looked up promoter regions for the gene and I did CpG island prediction to find islands. However, no CpG islands were found in the promoter region. Moreover, BSP primer design criteria entail that the primer pair must span a gene target with maximal number of islands without containing a CpG island in primer sequence itself.

 

question is:

 

Is there a use in looking at methylation status for gene expression inside the transcribed region? does DNA methylation outside promoter region affect gene transcription/expression?

Hi Moemedawar,

 

As the first, are you sure that the gene expression is regulated by the promoter methylation. Methylation independent primers primer can contain a limited number of CpGs ( look at Wojdacz et al; PMID: 17289753).






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