1 reply to this topic

[thanhtam]

Dear all,

I am trying purify a protein from inclusion body, so I prepared cell lysates under denative condition by adding urea 8M, but when I run cell lysates on SDS-PAGE, there is no band at all. I'm quite sure that the gel is fine since I checked it several times.

Did anyone has this problem? 

Please give me some advice

Thanks

[mdfenko]

how much protein are you loading in each lane?

 

how are you visualizing the lanes?

 

do you run standards? if so, how do they look?