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Problem with Denative lysis

Started by thanhtam, Dec 15 2013 10:06 AM

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#1 thanhtam

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Posted 15 December 2013 - 10:06 AM

Dear all,

I am trying purify a protein from inclusion body, so I prepared cell lysates under denative condition by adding urea 8M, but when I run cell lysates on SDS-PAGE, there is no band at all. I'm quite sure that the gel is fine since I checked it several times.

Did anyone has this problem? 

Please give me some advice

Thanks


#2 mdfenko

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Posted 16 December 2013 - 04:24 AM

how much protein are you loading in each lane?

 

how are you visualizing the lanes?

 

do you run standards? if so, how do they look?


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