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plasmid went missing

Started by cetok, May 12 2004 09:12 PM

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#1 cetok

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Posted 12 May 2004 - 09:12 PM

I did my plasmid miniprep and did also RE digestion to check my insert.
However, after sending it to sequencing...the plasmid went missing...
I check all my samples and it is what that happened...
what went wrong??

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#2 rassen

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Posted 13 May 2004 - 08:43 AM

Quote

after sending it to sequencing...the plasmid went missing

What do you mean by "the plasmid went missing"? What primer did you use for sequencing?

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#3 cetok

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Posted 16 May 2004 - 05:41 PM

i use m13f & r primer for sequencing..
anyway, i run gel on my samples..n it's true..
i can't find my plasmid n insert...
that's why i said it's misssing...hehehe

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#4 phdconsult

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Posted 30 May 2004 - 05:31 PM

Cetok

Did you use DNaseI to suspend your samplesby mistake? I am sure you are joking or are a really really tired lab worker- go get some sleep

Name: Don't Be Cheated
E-mail:dstf@doctor.com
Web: http://www.tait-services.com

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#5 cetok

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Posted 04 June 2004 - 09:36 AM

well...i'm sure i didn't put in DNase...but only Rnase , as i have no Dnase reagent... anyway, i think this topic should be closed by now..
so please no more replies....thks for looking into my question...sweet of u all...

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#6 cetok

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Posted 04 June 2004 - 09:37 AM

well...i'm sure i didn't put in DNase...but only Rnase , as i have no Dnase reagent... anyway, i think this topic should be closed by now..
so please no more replies....thks for looking into my question...sweet of u all... :rolleyes:

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