I did my plasmid miniprep and did also RE digestion to check my insert.
However, after sending it to sequencing...the plasmid went missing...
I check all my samples and it is what that happened...
what went wrong??
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5 replies to this topic
#2
rassen
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Posted 13 May 2004 - 08:43 AM
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after sending it to sequencing...the plasmid went missing
What do you mean by "the plasmid went missing"? What primer did you use for sequencing?
#3
cetok
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Posted 16 May 2004 - 05:41 PM
i use m13f & r primer for sequencing..
anyway, i run gel on my samples..n it's true..
i can't find my plasmid n insert...
that's why i said it's misssing...hehehe
anyway, i run gel on my samples..n it's true..
i can't find my plasmid n insert...
that's why i said it's misssing...hehehe
#4
phdconsult
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Posted 30 May 2004 - 05:31 PM
Cetok
Did you use DNaseI to suspend your samplesby mistake? I am sure you are joking or are a really really tired lab worker- go get some sleep
Did you use DNaseI to suspend your samplesby mistake? I am sure you are joking or are a really really tired lab worker- go get some sleep
#5
cetok
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Posted 04 June 2004 - 09:36 AM
well...i'm sure i didn't put in DNase...but only Rnase , as i have no Dnase reagent... anyway, i think this topic should be closed by now..
so please no more replies....thks for looking into my question...sweet of u all...
so please no more replies....thks for looking into my question...sweet of u all...
#6
cetok
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Posted 04 June 2004 - 09:37 AM
well...i'm sure i didn't put in DNase...but only Rnase , as i have no Dnase reagent... anyway, i think this topic should be closed by now..
so please no more replies....thks for looking into my question...sweet of u all...
so please no more replies....thks for looking into my question...sweet of u all...
