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Setting up reporter plasmid to measure promoter activity


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#1 tihong10

tihong10

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Posted 11 December 2013 - 07:34 PM

Hello,

 

I am new at using reporter plasmids so I've been running into some problems that want to present for advise...

 

My goal is to measure the activity of a bacterial promoter through fluorescence levels. So I am thinking this means that I need a fluorescent reporter vector that works in bacterial cells. I've come across reporter vectors are listed as being expressed in mammalian cells but also saw that they can propagate in E. coli (to make a plasmid stock). I'm wondering if I can use a vector that is listed as mammalian use to measure promoter activity in bacteria.

 

Which leads me to the question of replication of the plasmid in different types of bacteria. I am not sure about the whole origin compatibility issue so I can just ask what is the "origin of replication" that can work with Pseudomonas aeruginosa?

 

Thank you,

Tihong






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