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flag tag

tag flag N-terminus

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#1 M.B.T



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Posted 11 December 2013 - 02:52 PM

hi every body.



I come cross a gene with two different format one is gene +flag tag on N-terminus and the other is gene + flag tag on C-terminus.

I am wondering to know what is reason to have flag tag on C or N-terminus, and what's benefit of this sites.

I know that N-terminus is at begging of protein and C-terminus before stop codon of protein. 

is any one can help with this, thank u.


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Posted 11 December 2013 - 03:23 PM

Its hard to predict on which terminus the protein should be tagged; however selecting a 'tag or the terminus' are by considering that the inclusion of tag should'nt interfere with the biological function or the properties of the protein.  

I always had an alternate hypothesis....

#3 mdfenko


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Posted 12 December 2013 - 05:10 AM

and exposed for selection (as when purifying) in the form you require (native or denatured).

talent does what it can
genius does what it must
i used to do what i got paid to do

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