hi every body.
I come cross a gene with two different format one is gene +flag tag on N-terminus and the other is gene + flag tag on C-terminus.
I am wondering to know what is reason to have flag tag on C or N-terminus, and what's benefit of this sites.
I know that N-terminus is at begging of protein and C-terminus before stop codon of protein.
is any one can help with this, thank u.