So I recently joined a new lab. When I make gels, I microwave the agarose until there are no 'beads', immediately add 1/10,000 SYBR SAFE stain, let congeal, and then run the gel. However, every time I do this, and image the gel under a UV gel box, there's hardly any fluorescence. So what I have to do is post stain with SYBR (shake for 15 mins room temp) and it looks fine. I've always made gels the way I'm doing it now and it's worked fine. Any idea why I'm getting this weird result?
EDIT: I let gels congeal in the dark (usually within a drawer).
Edited by Ahrenhase, 11 December 2013 - 12:04 PM.