Edited by pellin, 11 December 2013 - 05:39 AM.
Asking for guidelines to start L6 cell line culturingL6 culture questions
Posted 11 December 2013 - 05:34 AM
Posted 11 December 2013 - 12:59 PM
I don't work with L6 cells, so I can't go into specifics. However, the advice on the ATCC website should be correct; they are the largest and most respected cell culture institution in the world and do know what they are doing.
The difference between FBS and calf serum is that FBS (fetal bovine serum) is harvested before the calf is born, so the components (mostly hormones, cytokines etc.) of the serum are different to those found in serum from animals that have been through the stress of birth, which results in massive hormonal and physiological changes.
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