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how to separate organelles from mammalian cells

separation organelles mammalian cell

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#1 Hellen

Hellen

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Posted 11 December 2013 - 03:30 AM

Hello everyone,

 

I want to get my target protein which is expressed in the CHO cells. With the help of GFP, I know my protein is localized in the nucleus membrane and ER membrane. Now I want to harvest my cells and separate the nucleus and ER from the cells.  I have no experience on this aspect. And I want to get your advices. Could you tell me some references or some useful websites or protocols to help me deal with this?

Thank you very much.

 



#2 Curtis

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Posted 11 December 2013 - 09:49 AM

look for cell fractionation protocols. 







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