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ATP hydrolysis

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#1 anonymous



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Posted 09 February 2001 - 10:00 PM

We are doing some ATP dependent enzyme assays. As a control for nonspecific reaction we substitute ATP with AMP. Unfortunately, we get a substantial amount of reaction going even with AMP, which we suspect that it is due to ATP contamination of the AMP. Does anybody have an easy method to deplete the ATP from the AMP, e.g.enzymatically with maybe a phosphatase? I would rather not go through the process of doing an HPLC purification. Or does anyone know of a source of AMP that is free of ATP?

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