Warning: array_merge(): Argument #2 is not an array in /home/protocol/public_html/forums/admin/sources/classes/text/parser.php on line 1074

Warning: Cannot modify header information - headers already sent by (output started at /home/protocol/public_html/forums/admin/sources/classes/text/parser.php:1074) in /home/protocol/public_html/forums/admin/sources/classes/output/formats/html/htmlOutput.php on line 110

Warning: Cannot modify header information - headers already sent by (output started at /home/protocol/public_html/forums/admin/sources/classes/text/parser.php:1074) in /home/protocol/public_html/forums/admin/sources/classes/output/formats/html/htmlOutput.php on line 127

Warning: Cannot modify header information - headers already sent by (output started at /home/protocol/public_html/forums/admin/sources/classes/text/parser.php:1074) in /home/protocol/public_html/forums/admin/sources/classes/output/formats/html/htmlOutput.php on line 136

Warning: Cannot modify header information - headers already sent by (output started at /home/protocol/public_html/forums/admin/sources/classes/text/parser.php:1074) in /home/protocol/public_html/forums/admin/sources/classes/output/formats/html/htmlOutput.php on line 137

Warning: Cannot modify header information - headers already sent by (output started at /home/protocol/public_html/forums/admin/sources/classes/text/parser.php:1074) in /home/protocol/public_html/forums/admin/sources/classes/output/formats/html/htmlOutput.php on line 141
ATP hydrolysis - Molecular Biology - BioForum

Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

ATP hydrolysis


  • Please log in to reply
No replies to this topic

#1 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
1
Neutral

Posted 09 February 2001 - 10:00 PM

We are doing some ATP dependent enzyme assays. As a control for nonspecific reaction we substitute ATP with AMP. Unfortunately, we get a substantial amount of reaction going even with AMP, which we suspect that it is due to ATP contamination of the AMP. Does anybody have an easy method to deplete the ATP from the AMP, e.g.enzymatically with maybe a phosphatase? I would rather not go through the process of doing an HPLC purification. Or does anyone know of a source of AMP that is free of ATP?




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.