I need to identify the gene/ suggest the function. I've been given a DNA sequence and I have to use BLAST (blastn). What I did so far (using the ncbi blast): i chose nucleotide blast program to run, entered my sequence, chose Others (nucleotide collection nr/nt) database and in the program selection part I picked Optimize for somewhat similar sequences (blastn). I got 100 hits. And now I'm unsure which one is the gene I'm looking for- the first hit? How do I determine so (the lowest e value, the highest ident, query cover)? I reckon it's triosephosphate isomerase...
I'd really appreciate your help, as I completely do not understand this programme and I have no idea what I'm doing but I do have find out the structure and function of a given sequence. If anyone could make that clear for me, would be forever in your debt.
This is the sequence: