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Help! How to remove dextran coated layer?

microbiology cell culture

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8 replies to this topic

#1 Celz

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Posted 09 December 2013 - 12:55 AM

Dear all, 

 

I am looking for a method to remove the dextran coated layer in order to release out the cells. Did anyone have any idea on it? 

 

Thanks. 



#2 El Crazy Xabi

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Posted 09 December 2013 - 04:45 PM

Enzymatic removal? --> Dextranase



#3 Celz

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Posted 09 December 2013 - 04:54 PM

How about other method? Such as heating or using acid?



#4 El Crazy Xabi

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Posted 09 December 2013 - 07:40 PM

You can check these:
http://onlinelibrary...020106/abstract

 

 

But be aware that acid digestion is not specific and may destroy what you want to recover.



#5 Celz

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Posted 09 December 2013 - 08:08 PM

I am just thinking what if I boil the sample in hot water, do you think maltodextrin will be broken down into small unit? 



#6 El Crazy Xabi

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Posted 09 December 2013 - 09:07 PM

Well, dextran is very heat-stable... what's the final purpouse of all this? YOu said to release cells but, do you want to use them for something afterwards?



#7 Celz

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Posted 09 December 2013 - 09:39 PM

Because the bacterial cells are being capsulated by maltodextrin, thus I am planning to look for a protocol to degrade down the maltodextrin in order to release out all the bacterial cells. 



#8 El Crazy Xabi

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Posted 09 December 2013 - 10:41 PM

Yes, I got that but, what do you want to use the cells for after that? Culturing, DNA extraction...?



#9 Celz

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Posted 09 December 2013 - 10:53 PM

Nope. I am planning to do for total cell count. 







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