this is nagu, asking some help regarding IL8 targetting in Western bloting
Every one is using ELISA for IL8.
But we had a antibodies, so i decided to do western bloting.
As I am using AGS cell line, it expresses IL8 and can be detected in ELISA.
But in western blotting i cant see the postive signal in both cell lysate and concentrated supernatant.
blocking 1 hour
Primary - 1:2000, over night
Secondary - 1: 3000 - 1 hour.
Washing 10 min - 3 times
can any one help me to out of my problem.
thanks in advance.