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How to design primer to amplify genomic DNA?
2 replies to this topic
Posted 05 December 2013 - 01:50 PM
I am designing primers which can amplify the genomic DNA on NCBI. Everything goes well until I have my primers. But when I put them in Blast, this sequence does not correspond at all to the gene I'm trying to study. What is wrong? Is it because the primer comes from the genome? Or do I make a mistake in the design? Can someone tell me how to design this kind of primer?
Thank you very much