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How many fibroblasts for 1ug DNA?


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#1 ramblingrat

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Posted 05 December 2013 - 12:42 PM

Hi,

 

Does anyone have an estimate, how many primary human fibroblast are needed to isolate 1ug of DNA? How many/which flasks of adherent cells do you normally harvest for 1ug of DNA?

I am normally a suspension-culture person, if anyone knew would be awesome!

 

Thanks a lot,

R.



#2 bob1

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Posted 05 December 2013 - 01:11 PM

According to Roche Lab FAQs, there is about 6 pg of total DNA per cell in a human cell, so I work that out to be about 166,667 cells.



#3 ramblingrat

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Posted 12 December 2013 - 10:33 AM

Oh - great! Thank you! :)






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