2 replies to this topic

[roxx_gurl]

Hi scientists,

 

 

Is there anyone by any chance include ascorbate in their media? I wanted to know what is difference between L-ascorbic acid and ascorbate in cell culture media? Is there any effect in term of growth of cell or one of them degrades quickly in incubater etc. 

 

Thanks

[bob1]

Chemically there shouldn't be any difference - ascorbate is the anion of ascorbic acid, which the acid will form when put into solution (C5H7O4COOH + H2O  goes to C5H7O4COO- + H+).  If you add something like sodium ascorbate, then you will also be adding sodium, which may not be a good idea in terms of osmolarity.

[roxx_gurl]

Thanks