Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

L-ascorbic acid Vs Ascorbate

culture media cell culture keratinocytes

  • Please log in to reply
2 replies to this topic

#1 roxx_gurl

roxx_gurl

    member

  • Active Members
  • Pip
  • 17 posts
3
Neutral

Posted 04 December 2013 - 10:42 PM

Hi scientists,

 

 

Is there anyone by any chance include ascorbate in their media? I wanted to know what is difference between L-ascorbic acid and ascorbate in cell culture media? Is there any effect in term of growth of cell or one of them degrades quickly in incubater etc. 

 

Thanks



#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,788 posts
406
Excellent

Posted 05 December 2013 - 12:07 PM

Chemically there shouldn't be any difference - ascorbate is the anion of ascorbic acid, which the acid will form when put into solution (C5H7O4COOH + H2O  goes to C5H7O4COO- + H+).  If you add something like sodium ascorbate, then you will also be adding sodium, which may not be a good idea in terms of osmolarity.



#3 roxx_gurl

roxx_gurl

    member

  • Active Members
  • Pip
  • 17 posts
3
Neutral

Posted 12 December 2013 - 09:49 AM

Thanks







Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.