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MTT T0 reading is higher than T48


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#1 EnglishBreakfast

EnglishBreakfast

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Posted 04 December 2013 - 02:59 PM

Hi,

 

I'm seeding 3500cells/well of HUVECs in 96 well plates and sometimes I get T0 readings higher than T48 readings, which doesn't make sense. I've tried this experiment so many times and it's always a 50% it works... Also, I am using VEGF to stimulate cell proliferation but it doesn't seem to give me a robust increase. Does any one have any ideas how to fix this? Any ideas would be very much appreciated.

 

Thanks






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