I'm cloning a digested insert to the digested vector (cut with same two restriction enzymes) which harbors Ap resistance. After transformation, is there any other way to confirm that I have a insert + vector clone than sequencing the purified plasmid or amplifying the insert with spesific primers?
I'm asking this because I assume that there will be also transformants on the Ap plates with the plain linearized vector which lacks the insert. The insert itself doesen't carry any selective marker. I have purified the linearized vector from agarose gel but I guess that I can't be sure that the sample contains only linearized form..? I don't want to proceed to the next step of the work unless I'm sure that I have a "right" transformant.
Edited by tmaz, 04 December 2013 - 07:16 AM.