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miRNA seed sequencing

miRNA

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#1 medbio

medbio

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Posted 04 December 2013 - 04:52 AM

Hi everyone,

I have some question about miRNAs. After making in silico analyze via TargetScan or the other web tools, I see 8mer, 7mer-m8. Which is the best matching? Also I see poorly conserved and highly conserved writings. Should I select the miRNA with highly conserved? or if I choose poorly conserved miRNA, is it false in theory?

After choosing miRNA, I want to do some functional assays such as qRT-PCR with miRNA\’ s target mRNA. In example hsa-miR-145 match with TPM3 (NM_001043351, tropomyosin 3) gene. When I design primers and prob for this gene, should I consider the miRNA macthing site? Should the primers and probe localized in miRNA:mRNA site?

Thanks for your attention

Best regards







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