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Primers

PCR Primers pM

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#1 Chris Black

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Posted 03 December 2013 - 01:10 PM

I really need some help.  I have 100uM Primer Stock.  I want my final concentration to be 4pmole/primer in a 25ul volume for a PCR reaction. Can you please help me?



#2 jerryshelly1

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Posted 03 December 2013 - 02:23 PM

1ul Primer (100uM) in 999uL water is 100nM. 

 

1uL primer (100nM) in 999uL water is 100pM.

 

Can you figure it out from here?







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