Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Primers mix


  • Please log in to reply
2 replies to this topic

#1 Paulo S Schlögl

Paulo S Schlögl

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 03 December 2013 - 10:02 AM

I am new to the PCR multiplex stuff and would like to know if its better to make a primer mix or work with any primer separately? I am working with genotyping (SNPs) of multi allelic genes?

Thanks



#2 Kasturi Borah

Kasturi Borah

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 03 December 2013 - 11:23 AM

Primer separately.



#3 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,504 posts
253
Excellent

Posted 03 December 2013 - 02:55 PM

Agree. Keeping the primers separate allow you to use them individually for sequencing, or as primers for a different PCR with a distinct second primer. You can prepare a dilute 10 uM mix, but keep the 100 uM stock primer separate.






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.