I'm trying to find a fluorochrome in the red channel that is suitable for both FACS and microscopy. Basically, I'm sorting cells using the Influx and then want to visually confirm they are what I think they are using a microscope afterward. Alexa 488 works well for the green channel but I'm having a bit of trouble finding something suitable for the red channel. I'm using an antibody clone that works in both FACS and IHC so I think it's only the fluorochrome that will be an issue. I know not all are suitable. For example, PE is great for FACS but photobleaches within seconds on the microscope. eFluor 650 seems to work in both but the data sheet from the antibodies explicitly say that they are not suitable for both techniques. Is this legitimate? Are there really differences in the antibody preparation if they are designed for FC vs IHC, assuming same clone, same fluorochrome, same company?
Thanks for any insights!