It could be a very stupid question, but just came in to my mind and thought of asking the best people in research . For storage of protein in -80, we add some % of glycerol in, so that the ice crystal could not destroy our protein. I was wondering, if it is possible to remove glycerol from protein by dialysis or some other method??
Then I thought people do sucrose gradient or glycerol gradient for various proteins separation. After visualizing on gel, do they separate protein from sucrose or glycerol?