Firstly..I'm new on this forum and I'm sorry if this question/problem is solved for several times on previous threads
I'm quite experienced with lab work but this is the first time when I counter this problem:
I've been running my digested vector on 1,2% gel with 1 x TBE. I intend to purify the linearized vector from the gel with commercial kit. Anyway.. for some reason all bands on the gel (including the ladder) appears to be very smeary. This has happened now for couple of times in a row. I run the gel 5V/cm (which should be just fine). Today when I was laying the gel on the UV-table I noticed that the gel was strangely warm. All my reagents should be fine, but I have some doubts with my TBE. Could "bad" running buffer somehow increase the current of the system resulting to the heated gel and degreaded (smeary?) samples ? My dye is Gelred.