I am working on Vitamin D receptor gene polymorphism using Fok I and Taq I and I am using, I am having trouble in determining PCR products sizes of (TT, Tt, tt, FF, Ff, ff) and the PCR marker/DNA ladder.
Taq I: 5’-CCT TCT TCT CTA TCC CCG TG-3’ (Forward) and 5’-GCA GGT CGG CTA GCT TCT-3’ (Reverse)
Fok I: 5’-ACT CTG GCT CTG ACC GTG-3’ (Forward) and 5’-TCA TAG CAT TGA AGT GAA AGC-3’
I have looked through too many articles, what should I do?
Thank you in advance