has any one had a problem where there are certain samples that reach the maximum threshold at the first cycle (ie, there is no amplification curve just a flatline at max levels).... but there is a product that is melting at the correct temperature?
To make matters a bit stranger... the samples which are looking "correct" have a melting curve that is about 10'C coller than normal... and are have a much later than normal ct?
This is a machine or software problem?
I have changed
re run samples that have previously worked
it is the same position for the max amplifications.... but still having everything else melting at a cooler temp... i have never had this happened before and it's driving me mad.