I need some advice - I'm going to be doing a double IF staining on PFA fixed cells. One antibody I have - anti rabbit... the other antibody I'm going to buy.
The problem is the best antibody is also anti rabbit.
There is an alternative that is anti mouse, but it has only been tested (and guaranteed) in IHC.
So... should I buy the anti mouse that may or may not give a result?
should i buy the anti rabbit and do a full stain with 1 ab, and then repeat with the second (which i haven't done and i'm not confident that it would work)?