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[Franz]

Hi!
In our lab we use the Qiagen Lambda Midi Kit for DNA extraction from phages. I created a genomic library using a Promega Kit, found a positive phage, amplified it and used this kit to elute DNA. My problems are: there always are DNAses in the eluted DNA in spite of the isopropanol precipitation step. The second problem is that after the inactivation of the DNAses, I cannot digest the DNA with either restriction enzyme. Who has some tips for me? Please help!!
Thanks
Franz