Hi all molecular biologists,
I will do western blot analysis.
but i still confuse in selecting the primary and secondary antibodies.
my sample is human fetal osteoblastic cell lines. I need to check for some protein.
for primary antibody, i would like to use anti osteocalcin , anti AP and anti integrin beta3.
1. how i suppose to write the primary antibody to order from company?
2. what are the secondary antibody i need to use?
thank you for helping me