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please i need clear steps and guide for bacterial enzyme extraction (sonication)

enzyme extraction purification protein concentration protein quantification

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#1 Erinle Kennie

Erinle Kennie


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Posted 26 November 2013 - 10:57 PM

Please, I isolated a Klebsiella oxytoca, and next step is to extract the esterase enzyme that degrades DMPhthalate ester. please I need a step by step approach and clear guide for the extraction, purification, protein quantification of the enzyme. I need to complete the practical before December ending and submit to my prof. thank you in advance. (most materials I got were not specific to volume of chemicals or which chemical specifically)

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